| Line Item Description: THIS IS A FIXED-COST AGREEMENT based upon the submission and approval of deliverables. See attached Scope of Work for details. The goals of this project are to characterize holobiont composition across host genetics, bacterial microbiome, and algal symbionts in nursery-reared coral populations, and to project populations persistence. T1: Quality Assurance Plan and Reporting ($3,819.08) T2: Microbiome Characterization of Broodstock and Juveniles via 16S Amplicon Sequencing ($22,401.95) T3: Symbiont Vertical Transmission and Stability Assessment via ITS2 Sequencing ($17,267.96) T4: Forward-Time Evolutionary Modeling of Nursery Population Thermal Tolerance ($13,369.28) T5: Cross-Species CBASS Thermal Stress Profiling of Nursery-Reared Juveniles ($43,955.88) T6: Genomic and Holobiont Characterization of 3-Plus-Year Montastraea cavernosa Outplants ($59,853.92) Q1 Deliverables Due on 9/28/26 ($7,746.85) T1 (15%) - QAP, Exhibit A, Exhibit G, Photo and summary T2 (5%) - Sample ID spreadsheet T3 (5%) - Sample ID spreadsheet T5 (5%) - Standardized CBASS heat stress protocol document, T6 (5%) - Spreadsheet of potential 3-plus-year M. cavernosa outplants available for sampling Q2 Deliverable Due on 2/1/27 ($10,299.39) T1 (10%) - Exhibit A Progress Report, Photo and summary for communication, all permits T2 (25%) - Confirmation of DNA extractions completed for all samples and initiation of library preparation T3 (25%) -Confirmation of DNA extractions completed for Year-1 samples and initiation of library preparation Q3 Deliverables Due on 3/30/27 ($62,626.17) T1 (0%) - Exhibit A Progress Report, Photo and summary for communication T2 (30%) - Sample ID spreadsheet with extraction, library prep metadata, and sequencing QC statistics T3 (30%) - Sample ID spreadsheet with extraction, library prep metadata, and sequencing QC statistics for Year-1 samples T4 (30%) - Summary report T5 (45%) - Sample ID spreadsheet, PAM fluorometry datasets for all coral species, CBASS tempera |
| Method of Procurement: Exempt, transaction with Governmental Entity or Governmental Agency [s. 287.057(3)(e)12, F.S.; defined in s. 287.012(14) and s. 163.3164(21), F.S.] |
Line Split Details:
| Split Accounting Line Number: 1 |
| FLAIR Contract ID: |
| Org. Code and Description: 37538010007 - |
| Object Code and Description: 139900 - |
| Account Code: 37-10-1-000286-37500400-00-100025-00 |
| Split Amount: $160,668.07 |
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