| Line Item Description: 321710 - ACD HybEZ II Hybridization System (115v)With ACD EZ-Batch Slide System - The HybEZ II Hybridization System (110V) allows hybridization and incubation of ACD's RNAscope assay. The oven holds up to 20 slides in a rack, and provides the necessary temperature control and humidity required for optimal performance of RNAscope manual assay. The system comprises: HybEZoven, a humidity control tray (PN 310012), and HybEZHumidifying Paper (2 sheets PN 310025), EZ-Batch Wash Tray (321717), EZ-Batch Slide Holder (321716). Cost is $5,420.00 322350 - The RNAscope 2.5 High Definition(HD)- RED Assay, is based on ACD's patented signal amplification and background suppression technology. The 2.5 HD version is a high sensitive RNA ISH technique and can be used for low expressing gene targets. The RNAscope 2.5 HD Reagent Kit-RED assay includes the Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope. Each kit contains three sub-kits: RNAscope 2.5 HD Detection Kit (RED) (Cat# 322360), RNAscope 2.5 Pretreat Reagents-H202 and Protease Plus (Cat#322330), RNAscope Target Retrieval (Cat#322000), RNAscope Wash Buffer (Cat#310091). Disclaimer: Photos for 2.5 products will be updated shortly. No cost. 439991 - RNAscope Probe - V-FV3-orf90R - Frog virus 3 major capsid protein gene complete cds No cost. 310043 - RNAscope Negative Control Probe - DapB - Bacillus subtilis strain SMY methylglyoxal synthase (mgsA) gene, partial cds dihydrodipicolinate reductase (dapB) gene, complete cds and YpjD (ypjD) gene, partial cds No cost 310018 - The hydrophobic barrier pen provides a heatstable water-repellent |
| Method of Procurement: Single source $2,500 or greater, not to exceed Category Two [Rule 60A-1.002(3), F.A.C.] |
Line Split Details:
| Split Accounting Line Number: 1 |
| FLAIR Contract ID: |
| Org. Code and Description: 77659060500 - |
| Object Code and Description: 519027 - |
| Account Code: 77-20-2-261002-77650200-00-109940-00 |
| Split Amount: $5,420.00 |
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